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KMID : 0984920140160020113
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Journal of Skin Barrier Research
2014 Volume.16 No. 2 p.113 ~ p.113
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Increased Expression of the Aryl Hydrocarbon Receptor in Patients with Chronic Inflammatory Skin Disease
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Kim Hye-One
Byun Yun-Sun
Kim Jin-Hye
Park Chun-Wook
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Abstract
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Background: Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that binds to various endogenous and exogenous ligands that are abundant in the skin. The aim of this study was to evaluate the significance of the AhR and its ligands in chronic inflammatory skin disease such as atopic dermatitis (AD) and psoriasis.
Methods: We recruited AD, psoriasis patients and healthy controls. The clinical severity was assessed and skin samples were taken. Real-time PCR, Immunohistochemical and immunofluorescence staining were performed in each skin sample. Cytokine expression profiles after treatment of normal human epidermal keratinocytes (NHEK) with TCDD or PCBs were measured. The serum levels of TCDD and PCBs were determined using high-resolution gas chromatography and mass spectrometry and both levels were higher in the AD group than in the control group.
Results: In the skin, expression of AhR and aryl hydrocarbon receptor nuclear translocator (ARNT) mRNA was increased in lesional skin from patients with AD and psoriasis compared to those of normal skin from healthy controls. The AhR and ARNT were co-localized in the nuclei of keratinocytes at the lower epidermis of psoriatic lesions, which suggested activation of the AhR pathway. After treatment of NHEKs with TCDD or PCBs, IL-6 and IL-8 production were increased. The serum levels of TCDD and PCBs were higher in the AD group than in the control group.
Conclusion: The results of this study suggest that the AhR is highly expressed in the acute lesional skin of AD and psoriasis patients and that activation of the AhR increases the expression of inflammatory cytokines in NHEK and may induce the development of chronic inflammatory skin diseases such as AD and psoriasis.
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KEYWORD
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